Journal
MOLECULAR BREEDING
Volume 31, Issue 1, Pages 27-37Publisher
SPRINGER
DOI: 10.1007/s11032-012-9765-0
Keywords
Allelic variation; Heading time; Photoperiodic response; Ppd-A1; Ppd-B1; Wheat
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Funding
- Ministry of Agriculture, Forestry and Fisheries of Japan [GD-3005]
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [23255007] Funding Source: KAKEN
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For genetic analysis of Ppd-1 homoeologs controlling photoperiodic response of wheat (Triticum aestivum L.), bulk segregant analysis was performed using a doubled haploid (DH) population derived from a cross of Japanese wheat genotypes Winter-Abukumawase and Chihokukomugi. Based on the segregation of simple sequence repeat markers linked to the Ppd-1 homoeologs, Winter-Abukumawase carried insensitive alleles Ppd-B1a and Ppd-D1a and Chihokukomugi carried a single insensitive allele (Ppd-A1a) that was first found in common wheat. The genomic sequence of Ppd-1 homoeologs including the 5' upstream region was determined and compared between the two genotypes. Ppd-D1a of Winter-Abukumawase had a deletion of 2,089 bp that was already reported for Ciano 67. Critical sequence polymorphism causing photoperiod insensitivity was not detected from the translation start codon to the 3' untranslated region of Ppd-A1 and Ppd-B1. However, novel mutations were found in the 5' upstream region. Ppd-A1a of Chihokukomugi had a deletion of 1,085 bp and Ppd-B1a of Winter-Abukumawase had an insertion of 308 bp. A total of 80 DH lines were classified into eight genotypes by PCR-based genotyping using specific primer sets to detect the In/Dels in the 5' upstream region of three Ppd-1 genes. The heading dates of the DH lines differed significantly between the eight genotypes, showing that each of the three insensitive alleles accelerates heading by 7-9 days compared with the photoperiod-sensitive genotype. Interaction between the three genes was also significant.
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