Journal
SCIENCE CHINA-CHEMISTRY
Volume 58, Issue 6, Pages 1056-1064Publisher
SCIENCE PRESS
DOI: 10.1007/s11426-014-5286-5
Keywords
magnetic microspheres; click chemistry; phenylboronic acid; enrichment; glycoproteins; glycopeptides
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Funding
- National Natural Science Foundation of China [21005018, 21375018, 21075016]
- National Basic Research Program of China [2010CB732403]
- National Science Foundation for Fostering Talents in Basic Research of China [J1103303]
- Doctoral Fund of Ministry of Education [20103514120002]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
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In this work, we demonstrate for the first time, a method to synthesize phenylboronic acid-Fe3O4@polydopamine (Fe3O4@PDA-PBA) magnetic microspheres via the combination of mussel-inspired polydopamine coating and click chemistry. Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microspheres with a core diameter of similar to 240 nm and a shell thickness of similar to 13 nm were obtained as identified by the characterization of the morphology, structure and composition of the synthesized microspheres. We evaluated the selectivity and binding capacity of the Fe3O4@PDA-PBA magnetic microspheres by using standard glycoproteins (ovalbumin, immunoglobulin G and catalase) and nonglycoproteins (human serum albumin, bovine hemoglobin, myoglobin, lysozyme, and ribonuclease A) as model proteins. Adsorption experiments, SDS-PAGE and mass spectrometry analysis demonstrated that the Fe3O4@PDA-PBA magnetic microspheres had much high binding capacity and selectivity for glycoproteins/glycopeptides compared to nonglycoproteins/nonglycopeptides. In addition, the practicability of the Fe3O4@PDA-PBA magnetic microspheres was further assessed by selective capture of glycoproteins from healthy human serum. The good results demonstrated its potential in glycoproteome analysis.
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