Journal
MOLECULAR BREEDING
Volume 30, Issue 1, Pages 441-451Publisher
SPRINGER
DOI: 10.1007/s11032-011-9633-3
Keywords
HVA1; Dhn6; Virus-induced gene silencing (VIGS); Barley stripe mosaic virus (BSMV); Water loss rate (WLR)
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Funding
- Chengdu Institute of Biology, Chinese Academy of Sciences
- Ministry of Agriculture, P. R. China [2008ZX08002-002]
- Ministry of Science and Technology of China [2006FY110700]
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Expression of the late embryogenesis abundant (LEA) gene is usually associated with plant response to dehydration. In this study, a drought-tolerant genotype was screened from 48 accessions of Tibetan hulless barley (Hordeum vulgare ssp. vulgare). By using virus-induced gene silencing, the influence of two LEA genes (HVA1 and Dhn6) on drought tolerance of Tibetan hulless barley was investigated. Results of quantitative real-time PCR indicated that the relative expression levels of HVA1 and Dhn6 in silenced plants were significantly reduced compared with control plants. Both HVA1-silenced and Dhn6-silenced plants showed a consequently lower survival rate than control plants under drought stress. However, only HVA1-silenced plants exhibited a significantly higher water loss rate (WLR). These results suggested that HVA1 and Dhn6 might participate in adaptive responses to water deficit in different ways. Vegetative growth of HVA1-silenced plants was significantly retarded even under optimal growth conditions, and their biomass accumulation was also much lower than that of the controls. These results indicate that HVA1 might play a role in vegetative growth of Tibetan hulless barley.
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