Journal
MOLECULAR BREEDING
Volume 23, Issue 1, Pages 153-161Publisher
SPRINGER
DOI: 10.1007/s11032-008-9222-2
Keywords
Marker assisted selection (MAS); Molecular marker; MFLP; Lupinus albus
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Funding
- Grain Research and Development Corporation (Australia) [DAW0102]
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Seeds and plants of wild type Lupinus albus are bitter and contain high level of alkaloids. During domestication, at least three genes conferring low-alkaloid content were identified and incorporated into commercial varieties. Australian lupin breeders exclusively utilize one of these sweetness genes, pauper'', in all varieties to prevent possible bitterness contamination via out-crossing. A cross was made between a sweet variety Kiev Mutant ( containing pauper gene) and a bitter type landrace P27174, and the population was advanced into F-8 recombinant inbred lines (RILs). Twenty-four plants representing sweetness and bitterness were subjected to DNA fingerprinting by the microsatellite-anchored fragment length polymorphism (MFLP) technique. A dominant polymorphism was discovered in an MFLP fingerprint. The MFLP marker was converted into a co-dominant, sequence-specific, simple PCR-based marker. Linkage analysis by the software program MapManager with marker score data and alkaloid phenotyping data from a segregating population containing 190 F-8 RILs indicated that the marker is linked to the pauper gene at the genetic distance of 1.4 centi-Morgans (cM). This marker, which is designated as PauperM1'', is capable of distinguishing the pauper gene from the other two low-alkaloid genes exiguus and nutricius. Validation on germplasm from the Australian lupin breeding program showed that the banding pattern of the marker PauperM1 is consistent with the alkaloid genotyping on a wide range of domesticated varieties and breeding lines. The PauperM1 marker is now being implemented for marker assisted selection in the Australian albus lupin breeding program.
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