Journal
MOLECULAR BIOSYSTEMS
Volume 8, Issue 3, Pages 726-729Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2mb05430b
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Funding
- Camille Dreyfus foundation
- [NSF-CHE0746446]
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Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS(center dot+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.
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