Journal
MOLECULAR BIOSYSTEMS
Volume 8, Issue 10, Pages 2513-2522Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2mb25100k
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Funding
- Japan Science and Technology Agency
- Special Coordination Fund for Promoting Sciences and Technology
- Ministry of Education, Culture, Sports, Science and Technology
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [23310140] Funding Source: KAKEN
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Robustness is one of the principles of design inherent to biological systems. Cellular robustness can be measured as limits of intracellular parameters such as gene expression levels. We have recently developed an experimental approach coined as genetic Tug-Of-War (gTOW), which we used to perform robustness analysis in yeast. Using gTOW, we were able to measure the upper limit of expression of gene targets. In this review, we first elaborate on how the gTOW method compares to current mathematical simulation models prevalently used in the determination of robustness. We then explain the experimental principles underlying gTOW and its associated tools, and we provide concrete examples of robustness analysis using gTOW, i.e. cell cycle and HOG pathway gene expression analysis. Finally, we list a series of Q&As related to the experimental utilization of gTOW and we describe the potential impact of gTOW and its relevance to the understanding of biological systems.
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