Journal
MOLECULAR BIOSYSTEMS
Volume 7, Issue 7, Pages 2114-2117Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1mb05047h
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Funding
- National Science Foundation [CHE-0644530]
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Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.
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