Journal
MOLECULAR BIOSYSTEMS
Volume 7, Issue 2, Pages 371-378Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0mb00109k
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Funding
- EU [NMP4-CT-2006-033231]
- SFI RFP [06/RFP/CHP031]
- EPA [2008-EH-MS-5-S3-R2]
- Irish Research Council for Science, Engineering and Technology
- Science Foundation Ireland [SFI/SRC/B1155]
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A spatio-temporal mapping of the uptake of silica (SiO2) nanoparticles of different sizes by lung epithelial cells has been obtained. Based on high control of nanoparticle dispersion in cell media and cell exposure, one obtains reproducible and quantitative time-resolved data using a combination of flow cytometry, fluorescence and electron microscopies. We are thereby able to give a rather detailed account of the journey of SiO2 nanoparticles from the early events of uptake to their final sub-cellular localization.
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