Journal
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
Volume 16, Issue 6, Pages -Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1088/1468-6996/16/6/065004
Keywords
iridium(III); exonuclease III; G-quadruplex; mercury(II) ion
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Funding
- Hong Kong Baptist University [FRG2/14-15/004]
- Health and Medical Research Fund [HMRF/13121482, HMRF/14130522]
- Research Grants Council [HKBU/201811, HKBU/204612, HKBU/201913]
- French National Research Agency/Research Grants Council Joint Research Scheme [A-HKBU201/12]
- State Key Laboratory of Environmental and Biological Analysis Research Grant [SKLP-14-15-P001]
- National Natural Science Foundation of China [21575121]
- Guangdong Province Natural Science Foundation [2015A030313816]
- Hong Kong Baptist University Century Club Sponsorship Scheme
- Interdisciplinary Research Matching Scheme [RC-IRMS/14-15/06]
- State Key Laboratory of Synthetic Chemistry
- Science and Technology Development Fund, Macao SAR [098/2014/A2, 007/2014/AMJ]
- University of Macau [MYRG091(Y3-L2)-ICMS-12-LCH, MYRG2015-00137-ICMS-QRCM, MRG023/LCH/2013/ICMS]
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We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+.
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