Journal
MOLECULAR BIOSYSTEMS
Volume 7, Issue 4, Pages 1056-1059Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0mb00338g
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Funding
- National Academies Keck Futures Initiative on Synthetic Biology [NAKFI SB13]
- National Institutes of Health [GM077596]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P01GM077596] Funding Source: NIH RePORTER
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We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.
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