Fibroblast growth factor 10 (FGF10) promotes the adipogenesis of intramuscular preadipocytes in goat

Fibroblast growth factor 10 (FGF10) is an adipokine that is found to participate in the regulation of adipogenesis. However, its function remains to be elucidated in intramuscular fat (IMF) deposition of goat. The purpose of this study was to explore the role of FGF10 in goat IMF deposition. Here, we investigated the expression of FGF10 in goat intramuscular adipocytes inducing 0, 2, 4, 6 and 8 days. Effect of FGF10 on adipogenesis was investigated by gaining and losing function of FGF10 in vitro. And then, we examined several lipid metabolism-related genes, including peroxisome proliferator activated receptor gamma (PPAR gamma), sterol regulatory element binding protein 1 (SREBP1), preadipocyte factor-1 (Pref-1), CCAAT/enhancer binding protein-alpha (C/EBP alpha) and CCAAT/enhancer binding protein-beta (C/EBP beta), as well as, Kruppel-like factor (KLF) family. We found that the sharp expression of FGF10 appeared at 2 days. Overexpression of FGF10 mediated by adenovirus promotes lipid accumulation, accompanied by up-regulating of LPL and C/EBP alpha with the down-regulating of C/EBP beta. Conversely, the expression of LPL, C/EBP alpha and SREBP1 was significantly decreased by the siRNAs of FGF10. Meanwhile, we showed that FGF10 regulated the expression of many KLFs members and interacted synergistically or antagonistically with them. Thus, our results demonstrated a key role of FGF10 as a positively factor in the regulation of adipogenic differentiation of intramuscular preadipocyte in goat.