Journal
MOLECULAR BIOLOGY REPORTS
Volume 41, Issue 3, Pages 1437-1444Publisher
SPRINGER
DOI: 10.1007/s11033-013-2988-2
Keywords
FcIMD; Innate immunity; IMD pathway; AMPs
Categories
Funding
- National Natural Science Foundation of China [31072203, 31272683]
- Major State Basic Research Development Program of China (973 program) [2012CB114403]
- China Agriculture Research system [CARS-47]
Ask authors/readers for more resources
IMD pathway is an important signaling pathway in regulating the innate immunity of invertebrate. IMD is regarded as a key adaptor to link the extracellular signals and intracellular signals. In the present study, a full-length cDNA encoding an IMD homolog (FcIMD) was isolated from Chinese shrimp Fenneropenaeus chinensis. It consists of 767 bp with a 483 bp open reading frame encoding 160 amino acids. A death domain was predicted which is crucial to the immune function of IMD. Tissue expression analysis showed that FcIMD mRNA had different expression level in different tissues. The transcriptional expression of FcIMD in shrimp was significantly induced at early stage (1 h) after stimulation by Vibrio or Micrococcus which suggested its important role in the immunity of shrimp to bacterium infection. The expressions of shrimp antimicrobial peptides (AMPs) were affected when FcIMD was silenced by dsRNA interference. Through analyzing the expression of FcRelish, FcDorsal and FcJNK in FcIMD silenced shrimp at 1 h post Vibrio injection, we deduced that IMD was involved in Relish regulated signaling pathway, not in Dorsal or JNK regulated signaling pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available