Association between subcutaneous and intramuscular fat content in porcine ham and loin depending on age, breed and FABP3 and LEPR genes transcript abundance

The objective of the present study was to analyze the level of intramuscular fat (IMF) in loin (musculus longissimus dorsi) and ham (musculus semimembranosus) and the level of subcutaneous fat in these cuts depending on breed, age and the expression level of FABP3 and LEPR genes. The results obtained showed that only the breed influenced on the level of both intramuscular and subcutaneous fat to the same extent (P a parts per thousand currency sign 0.001). The age of animals had an effect on fat content of the cuts (P a parts per thousand currency sign 0.001) and to a lower extent on the level of IMF in both muscles (P a parts per thousand currency sign 0.05). We confirmed highly significant effect of breed and age on the LEPR mRNA abundance-the expression of the this gene increased significantly (P a parts per thousand currency sign 0.01) with age and the highest expression was found for the Puawska breed in m. longissimus dorsi and for the Polish Landrace breed in m. semimembranosus. We observed the high correlations between the transcript level of the LEPR gene and the fat content of individual cuts (P a parts per thousand currency sign 0.01). The expression level of FABP3 gene influenced the level of IMF (P a parts per thousand currency sign 0.01), but not the level of subcutaneous fat in loin and ham.