Journal
MOLECULAR BIOLOGY REPORTS
Volume 40, Issue 4, Pages 2907-2914Publisher
SPRINGER
DOI: 10.1007/s11033-012-2306-4
Keywords
FATP1; Chicken preadipocyte; Apoptosis; Cell differentiation; Fat metabolism
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Funding
- National Nature Science Foundation of China [30871785, 31172185]
- Program for New Century Excellent Talents in Universities, Chinese Ministry of Education [NCET-06-0865]
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FATP1 plays an important role in the trafficking of free fatty acids in adipocytes, however, its precise function and relationship with other fatty acid transporters all remain poorly understood. In this study, FATP1 gene silencing was induced by transfecting siRNA of target sequence into chicken preadipocytes, then the expression of FABP was found down-regulated while the expression of FAT was raised. In addition, differential inhibition of the cells was observed and the expressions of PPAR gamma and C/EBP alpha were found down-regulated. Moreover, the silencing also induced the down-regulation of FAS and inhibited the adipogenesis in adipocytes. Of specific interest here was that FATP1 silencing significantly improved the expressions and activities of cell apoptotic factors Caspases 3 and BCL2 associated X protein (Bax). Consequently, FATP1 deficiency prevented the differentiation while induced apoptosis in chicken preadipocytes.
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