Journal
MOLECULAR BIOLOGY REPORTS
Volume 39, Issue 7, Pages 7635-7646Publisher
SPRINGER
DOI: 10.1007/s11033-012-1598-8
Keywords
Selenate-responsive gene; Selenite-responsive gene; Se-hyperaccumulating plant; Fluorescent differential display
Categories
Funding
- United States Department of Agriculture-Cooperative State Research, Education, and Extension Service [2009-35318-05032]
- North Carolina Biotechnology Center [2007-BRG-1223]
- Golden LEAF Foundation
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Plants with capacity to accumulate high levels of selenium (Se) are desired for phytoremediation and biofortification. Plants of genus Astragalus accumulate and tolerate high levels of Se, but their slow growth, low biomass and non-edible properties limit their direct utilization. Genetic engineering may be an alternative way to produce edible or high biomass Se-accumulating plants. The first step towards this goal is to isolate genes that are responsible for Se accumulation and tolerance. Later, these genes can be introduced into other edible and high biomass plants. In the present study, we applied fluorescent differential display to analyze the transcript profile of Se-hyperaccumulator A. racemosus treated with 20 mu M selenate (K2SeO4) for 2 weeks. Among 125 identified Se-responsive candidate genes, the expression levels of nine were induced or suppressed more than twofold by selenate treatment in two independent experiments while 14 showed such changes when treated with selenite (K2SeO3). Six of them were found to respond to both selenate and selenite treatments. A novel gene CEJ367 was found to be highly induced by both selenate (1,920-fold) and selenite (579-fold). Root- or shoot-preferential expression of nine genes was further investigated. These identified genes may allow us to create Se-enriched transgenic plants.
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