Journal
MOLECULAR BIOLOGY REPORTS
Volume 40, Issue 2, Pages 957-967Publisher
SPRINGER
DOI: 10.1007/s11033-012-2137-3
Keywords
Potato; Late blight; Nicotiana benthamiana; Quantitative resistance; Virus-induced gene silencing; Durable resistance
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Funding
- Natural Science Foundation of Hubei Province [2010CDA102]
- Earmarked Fund for Modern Agro-industry Technology Research System [CARS-10-P06]
- China Scholarship Council Program for Graduate Students
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The most significant threat to potato production worldwide is the late blight disease, which is caused by the oomycete pathogen Phytophthora infestans. Based on previous cDNA microarrays and cDNA-amplified fragment length polymorphism analysis, 63 candidate genes that are expected to contribute to developing a durable resistance to late blight were selected for further functional analysis. We performed virus-induced gene silencing (VIGS) to these candidate genes on both Nicotiana benthamiana and potato, subsequently inoculated detached leaves and assessed the resistance level. Ten genes decreased the resistance to P. infestans after VIGS treatment. Among those, a lipoxygenase (LOX; EC 1.13.11.12) and a suberization-associated anionic peroxidase affected the resistance in both N. benthamiana and potato. Our results identify genes that may play a role in quantitative resistance mechanisms to late blight.
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