Journal
MOLECULAR BIOLOGY REPORTS
Volume 39, Issue 4, Pages 4553-4564Publisher
SPRINGER
DOI: 10.1007/s11033-011-1245-9
Keywords
Pak-choi; ABA; Abiotic stress; WRKY; Overexpression
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Funding
- earmarked fund for Modern Agro-industry Technology Research System [Ny-cytx-35-gw12]
- National Science and Technology Support Program [2009BADB8B03-1]
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WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced quickly and strongly in response to various environmental stresses, including low temperatures, ABA, salt and dehydration. Constitutive expression of BcWRKY46 in tobacco under the control of the CaMV35S promoter reduced the susceptibility of transgenic tobacco to freezing, ABA, salt and dehydration stresses. Our studies suggest that BcWRKY46 plays an important role in responding to ABA and abiotic stress.
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