4.5 Article

Analysis of gene expression profile of Arabidopsis genes under trichloroethylene stresses with the use of a full-length cDNA microarray

Journal

MOLECULAR BIOLOGY REPORTS
Volume 39, Issue 4, Pages 3799-3806

Publisher

SPRINGER
DOI: 10.1007/s11033-011-1157-8

Keywords

cDNA Microarray; Trichloroethylene; Arabidopsis thaliana; Phytoremediation; Gene expression

Funding

  1. Hi-tech Research and Development Program (863) of China [2008AA 10Z401]
  2. Shanghai Natural Science Foundation [11ZR 1432600]

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Trichloroethylene (TCE) is a widespread and persistent environmental contaminant. Plants are able to take up a range of harmful organic compounds, including some of the most abundant environmental pollutants like TCE. In this study, complementary DNA microarrays were constructed to have a better view of transcript expression in Arabidopsis thaliana during TCE-induced stress. The microarray analysis demonstrated the complexity of gene expression patterns resulting from TCE. A total of 1,020 transcripts were differentially up-regulated by TCE. Those genes might specifically contribute to the TCE transformation, conjugation, and compartmentation in plant. This study provides informative preliminary data for more in-depth analyses of TCE tolerance in Arabidopsis thaliana.

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