Journal
MOLECULAR BIOLOGY REPORTS
Volume 39, Issue 2, Pages 1527-1535Publisher
SPRINGER
DOI: 10.1007/s11033-011-0891-2
Keywords
Late-flowering phenotype; Quantitative RT-PCR; Stomatal closure; Seed germination
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Funding
- National Natural Science Foundation of China [30770200, 30871325, 31071076]
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The homozygous T-DNA mutants of AtCAL1 (Rat1) and AtCAL2 (Rat2) were obtained. The double mutant of Rat2/Rat1RNAi was constructed which showed obvious late-flowering phenotype from others. The expression of various flowering-related genes was studied among mutants and wild-type plants by quantitative RTPCR. The double mutant plants showed the shortest root length compared with T-DNA insertion mutants and wild type plants under red light, blue light, and white light. The double mutants showed hypersensitivity to NaCl and ABA. However, these mutants had no effect on stomatal closure by ABA.
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