Journal
MOLECULAR BIOLOGY REPORTS
Volume 38, Issue 8, Pages 5179-5183Publisher
SPRINGER
DOI: 10.1007/s11033-010-0667-0
Keywords
Cattle; CACNA2D1 gene; Somatic cell count; Somatic cell score; Mastitis
Categories
Funding
- earmarked fund for Modern Agro-industry Technology Research System [nycytx-38]
- Eleventh Five-Year National Science and Technology Support Project [2006BAD04A16, 2007BAD56B04]
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The objective of the present study was to identify polymorphisms of the CACNA2D1 gene, and to analyze associations between these polymorphisms and mastitis in several cattle breeds. Through PCR-RFLP methods and DNA sequencing, an allelic variant corresponding to the A -> G mutations and Aspartic (Asp) to Glycine (Gly) amino acid replacement at positions 526745 in the exon 25 of bovine CACNA2D1 gene could be detected. Two alleles, A and G, and three genotypes, AA, AG and GG were defined. Genetic character in the studied populations indicated that the A526745G loci of CACNA2D1 gene was moderate polymorphism and fitted with Hardy-Weinberg equilibrium (P > 0.05). The effects of CACNA2D1 polymorphisms on somatic cell score (SCS) were analyzed and significant association was found between A526745G and SCS. The mean of genotype GG was significantly lower than those of genotype AG and AA (P = 0.0469). Information provided in this research could be useful in further studies to determine the role of CACNA2D1 gene in the mastitis resistance.
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