Journal
MOLECULAR BIOLOGY REPORTS
Volume 38, Issue 5, Pages 3023-3028Publisher
SPRINGER
DOI: 10.1007/s11033-010-9968-6
Keywords
KAP7.1; KAP8.2; Hair follicles; In situ hybridization; Quantitative real-time PCR; Liaoning new-breeding cashmere goat
Categories
Funding
- National Natural Science Foundation of China [30571324, 30972079]
- Natural Science Fund of Liaoning Province [20072151]
- Science and technology plan project of Dalian [2008B12NC079]
Ask authors/readers for more resources
Keratin-associated protein is one of the major structural proteins of the hair, whose content in hair has important effect on the quality of cashmere. In order to study the relationship between HGTKAP gene expression and cashmere fineness, the quantitative real-time RT-PCR (qRT-PCR) was firstly used to detect the levels of KAP7.1, KAP8.2 gene expression in the primary and secondary hair follicles; semi-quantitative RT-PCR was used to detect whether KAP7.1, KAP8.2 gene are expressed in heart, liver, spleen, lung, kidney tissues; and in situ hybridization(ISH) to detect KAP7.1 gene expression location. qRT-PCR result showed that the expression of both KAP7.1 and KAP8.2 gene in the secondary hair follicles are significantly higher than that in the primary follicles, relative quantitative analysis obtained that KAP7.1 was 2.28 times, while KAP8.2 was 2.71 times. Semi-quantitative RT-PCR results revealed that KAP 7.1 and KAP8.2 mRNA were not detected in the heart, liver, spleen, lung and kidney tissues, demonstrating that KAP7.1 and KAP8.2 were specially expressed in hair follicles, participating in hair formation. Moreover, KAP7.1 gene has a strong expression in the cortical layer, inner root sheath of the primary follicles and the cortical layer, inner root sheath and hair matrix of the secondary hair follicles by ISH analysis. Taken together, the evidence presented here indicated that in the formation of cashmere and wool, differential expression of these two genes in the primary and secondary hair follicles may have an important role in regulating the fiber diameter.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available