Journal
MOLECULAR BIOLOGY REPORTS
Volume 37, Issue 8, Pages 3735-3745Publisher
SPRINGER
DOI: 10.1007/s11033-010-0027-0
Keywords
Fe-excess; Representational difference analysis; Rice; Stress; WRKY transcription factor
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Funding
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil) [481131/2004-3]
- HarvestPlus [6005-05]
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil)
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Fe participates in several important reactions in plant metabolism. However, Fe homeostasis in plants is not completely understood, and molecular studies on Fe-excess stress are scarce. Rice (Oryza sativa L. ssp. indica) is largely cultivated in submerged conditions, where the extremely reductive environment can lead to severe Fe overload. In this work, we used representational difference analysis (RDA) to isolate sequences up-regulated in rice shoots after exposure to Fe-excess. We isolated 24 sequences which have putative functions in distinct cellular processes, such as transcription regulation (OsWRKY80), stress response (OsGAP1, DEAD-BOX RNA helicase), proteolysis (oryzain-alpha, rhomboid protein), photosynthesis (chlorophyll a/b binding protein), sugar metabolism (beta glucosidase) and electron transport (NADH ubiquinone oxireductase). We show that the putative WRKY transcription factor OsWRKY80 is up-regulated in rice leaves, stems and roots after Fe-excess treatment. This up-regulation is also observed after dark-induced senescence and drought stress, indicating that OsWRKY80 could be a general stress-responsive gene. To our knowledge, this is the first report of an Fe-excess-induced transcription factor in plants.
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