Journal
MOLECULAR BIOLOGY REPORTS
Volume 37, Issue 8, Pages 4067-4073Publisher
SPRINGER
DOI: 10.1007/s11033-010-0066-6
Keywords
Gene structure; Maize; Mitogen-activated protein kinase; Salt stress; Transgenic Arabidopsis
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Funding
- National Natural Science Foundation of China [30471052, 30871457]
- State Key Basic Research and Development Plan of China [2009CB118500]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0635]
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Mitogen-activated protein kinase (MAPK) cascades play a remarkably crucial role in plants. It has been studied intensively in model plants Arabidopsis, tobacco and rice. However, the function of MAPKs in maize (Zea mays L.) has not been well documented. ZmSIMK1 (Zea mays salt-induced mitogen-activated protein kinase 1) is a previously identified MAPK gene in maize. In this research, we charactered ZmSIMK1 and showed that ZmSIMK1 was involved in Arabidopsis salt stress. The genomic organization of ZmSIMK1 gene and its expression in maize have been analyzed. In order to investigate the function of ZmSIMK1, we generated transgenic Arabidopsis constitutively overexpressing ZmSIMK1. Ectopic expression of ZmSIMK1 in Arabidopsis resulted in increased resistance against salt stress. Importantly, ZmSIMK1-overexpressing Arabidopsis exhibited constitutive expression of stress-responsive marker genes, RD29A and P5CS1. Furthermore, RD29A and P5CS1 were upregulated under salt stress. These results suggest that ZmSIMK1 may play an important role in plant salt stress.
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