Journal
MOLECULAR BIOLOGY REPORTS
Volume 38, Issue 6, Pages 3605-3613Publisher
SPRINGER
DOI: 10.1007/s11033-010-0472-9
Keywords
Iron; Ferrous iron; Ferric chelate reductase; FRO6; Chlorophyll; Transgenic tobacco
Categories
Funding
- National Natural Science Foundation [30970552]
- Foundation for Key Teachers from Heilongjiang Educational Committee [1005HQ024]
- Natural Science Foundation of Heilongjiang Province of China [C2005-07]
- Scientific Research Foundation for Returned Scholars, Ministry of Education of China
Ask authors/readers for more resources
The Arabidopsis gene FRO6(AtFRO6) encodes ferric chelate reductase and highly expressed in green tissues of plants. We have expressed the gene AtFRO6 under the control of a 35S promoter in transgenic tobacco plants. High-level expression of AtFRO6 in transgenic plants was confirmed by northern blot analysis. Ferric reductase activity in leaves of transgenic plants grown under iron-sufficient or iron-deficient conditions is 2.13 and 1.26 fold higher than in control plants respectively. The enhanced ferric reductase activity led to increased concentrations of ferrous iron and chlorophyll, and reduced the iron deficiency chlorosis in the transgenic plants, compared to the control plants. In roots, the concentration of ferrous iron and ferric reductase activity were not significantly different in the transgenic plants compared to the control plants. These results suggest that FRO6 functions as a ferric chelate reductase for iron uptake by leaf cells, and overexpression of AtFRO6 in transgenic plants can reduce iron deficiency chlorosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available