Journal
MOLECULAR BIOLOGY REPORTS
Volume 37, Issue 1, Pages 473-476Publisher
SPRINGER
DOI: 10.1007/s11033-009-9654-8
Keywords
Bulls; CAPNS1 gene; SNP; Gene polymorphism; SSCP; PCR-RFLP
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Funding
- Ministry of Scientific Research and Information Technology [3 P06D01825, N N311 034834]
- IGHZ project
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Calpains are a ubiquitous cytoplasmic cysteine protease, the activity of which is absolutely dependent on calcium. This proteolytic system degrades myofibrillar protein under post-mortem conditions and appears to be the primary enzyme in the postmortem tenderization process. In the present study a new single nucleotide polymorphism was found in the bovine CAPNS1 gene exon 11 coding for the 3'UTR. Transition C -> T at position 6536 was detected and identified using PCR-SSCP and DNA sequencing techniques, and then analysed with PCR-RFLP using MboII nuclease. The genotype frequencies and alleles distribution were studied in 190 bulls including, Charolaise, Hereford, Limousine, Simmental, Polish Red and Fresian breeds.
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