Journal
MOLECULAR BIOLOGY REPORTS
Volume 37, Issue 2, Pages 717-727Publisher
SPRINGER
DOI: 10.1007/s11033-009-9570-y
Keywords
Phytophthora capsici; Elicitin; Capsicein; HR; RLK; NgRLK1
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Funding
- National Institute of Agricultural Biotechnology, Rural Development Administration, Republic of Korea
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Elicitins, extracellular proteins from Phytophthora fungi, elicit a hypersensitivity response (HR), including systemic acquired resistance, in some plants. The elicitin capsicein (similar to 10 kDa) was purified by FPLC from culture filtrates of P. capsici. Purified native and recombinant capsicein induced a hypersensitive response in leaves of the non-host plants Nicotiana glutinosa and Brassica rapa subsp. pekinensis. To search for candidate capsicein-interacting proteins from N. glutinosa, a yeast two-hybrid assay was used. We identified a protein interactor that is homologous to a serine/threonine kinase of the plant receptor-like kinase (RLK) group and designated it NgRLK1. The ORF of NgRLK1 encodes a polypeptide of 832 amino acids (93,490 Da). A conserved domain analysis revealed that NgRLK1 has structural features typical of a plant RLK. NgRLK1 was autophosphorylated, with higher activity in the presence of Mn2+ than Mg2+.
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