Journal
MOLECULAR BIOLOGY REPORTS
Volume 37, Issue 3, Pages 1335-1340Publisher
SPRINGER
DOI: 10.1007/s11033-009-9512-8
Keywords
Estrogen receptor alpha; Fusion protein; Protein purification; Overproduction; Induction; Lysate
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Funding
- University Grants Commission, India
- Department of Biotechnology, Govt. of India [BT/PR3593/Med/14/468/2003]
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Escherichia coli BL21 (DE3) is commonly used for the overproduction of fusion proteins. Using this system, we recently reported the overproduction of histidine-tagged mouse estrogen receptor (ER) alpha-ligand binding domain as an intact 30 kD protein and its inhibitory effect on the growth of bacteria. However, when GST-tagged mouse ER alpha transactivation domain (TAD) was overproduced using this system, it showed no effect on the growth of bacteria but was specifically degraded during its expression and purification. Here we report the expression of 47 kD GST-tagged mouse ER alpha-TAD protein, which was degraded partially and specifically into 46 and 43 kD fragments. This fusion protein was further degraded into 37, 31, 29 and 26 kD fragments during its purification by affinity chromatography. Such specific degradation of GST-tagged mouse ER alpha-TAD during its overproduction in E. coli and purification indicates the induction of specific protease and suggests the modification of expression system.
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