Journal
MOLECULAR BIOLOGY REPORTS
Volume 36, Issue 7, Pages 1647-1650Publisher
SPRINGER
DOI: 10.1007/s11033-008-9364-7
Keywords
HDV ribozyme; Reaction mechanism; High hydrostatic pressure (HHP)
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Funding
- Scientific Network of Institute of Organic Chemistry
- Institute of Bioorganic Chemistry, Polish Academy of Sciences
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The hepatitis delta virus (HDV) ribozyme is an RNA enzyme that catalyzes the site-specific trans-esterification reaction. Using high hydrostatic pressure (HHP) technique we showed that HDV ribozyme catalyzes the reaction of RNA cleavage in the absence of magnesium ions according to mechanism of acidic hydrolysis of esters. HHP induces changes of water structure, lowering pH and effect ribozyme catalytic site structure formation without magnesium. HHP, similarly to magnesium ion at ambient pressure stabilizes the higher order RNA structure of HDV, but Mg2+ is not involved in the catalysis. Our results clearly support the new mechanism of HDV hydrolysis and show advantages of using HHP in analysis of macromolecules interaction.
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