Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 25, Issue 10, Pages 1545-1548Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E13-05-0249
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Funding
- Elizabeth Clay Howald Presidential Fellowship from The Ohio State University
- National Institutes of Health [R01GM086546]
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Protein numbers in cells determine rates of biological processes, influence the architecture of cellular structures, reveal the stoichiometries of protein complexes, guide in vitro biochemical reconstitutions, and provide parameter values for mathematical modeling. The purpose of this essay is to increase awareness of methods for counting protein molecules using fluorescence microscopy and encourage more cell biologists to report these numbers. We address the state of the field in terms of utility and accuracy of the numbers reported and point readers to references for details of specific techniques and applications.
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