Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 25, Issue 19, Pages 2934-2947Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E14-05-0957
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Funding
- National Science Foundation [MCB-0718384, MCB-1121771]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1121771] Funding Source: National Science Foundation
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During meiosis, homologues are linked by crossover, which is required for bipolar chromosome orientation before chromosome segregation at anaphase I. The repetitive ribosomal DNA (rDNA) array, however, undergoes little or no meiotic recombination. Hyper-recombination can cause chromosome missegregation and rDNA copy number instability. We report here that condensin, a conserved protein complex required for chromosome organization, regulates double-strand break (DSB) formation and repair at the rDNA gene cluster during meiosis in budding yeast. Condensin is highly enriched at the rDNA region during prophase I, released at the prophase I/metaphase I transition, and reassociates with rDNA before anaphase I onset. We show that condensin plays a dual role in maintaining rDNA stability: it suppresses the formation of Spo11-mediated rDNA breaks, and it promotes DSB processing to ensure proper chromosome segregation. Condensin is unnecessary for the export of rDNA breaks outside the nucleolus but required for timely repair of meiotic DSBs. Our work reveals that condensin coordinates meiotic recombination with chromosome segregation at the repetitive rDNA sequence, thereby maintaining genome integrity.
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