Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 25, Issue 7, Pages 992-1009Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E13-08-0506
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Funding
- National Institutes of Health [R01-GM033279, R01-GM069909]
- Welch Foundation Grant [I-1532]
- UT Southwestern Endowed Scholars Program
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The nuclear import receptors importin beta and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin beta is known to act by repressing assembly factors in regions distant from chromatin, whereas RanGTP produced on chromatin frees factors from importin beta for localized assembly. The mechanism of transportin regulation was unknown. Diametrically opposed models for transportin action are as follows: 1) indirect action by RanGTP sequestration, thus down-regulating release of assembly factors from importin beta, and 2) direct action by transportin binding and inhibiting assembly factors. Experiments in Xenopus assembly extracts with M9M, a super-affinity nuclear localization sequence that displaces cargoes bound by transportin, or TLB, a mutant transportin that can bind cargo and RanGTP simultaneously, support direct inhibition. Consistently, simple addition of M9M to mitotic cytosol induces microtubule aster assembly. ELYS and the nucleoporin 107-160 complex, components of mitotic kinetochores and nuclear pores, are blocked from binding to kinetochores in vitro by transportin, a block reversible by M9M. In vivo, 30% of M9M-transfected cells have spindle/cytokinesis defects. We conclude that the cell contains importin beta and transportin global positioning systemor GPS pathways that are mechanistically parallel.
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