Journal
SCIENCE
Volume 347, Issue 6229, Pages 1446-1452Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa5372
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Funding
- European Research Council [256270]
- Deutsche Forschungsgemeinschaft
- NIM (Nanosystems Initiative Munich)
- Technische Universitat Munchen (TUM) Institute for Advanced Study
- TUM International Graduate School of Science and Engineering (IGSSE)
- [Sonderforschungsbereich SFB863]
- European Research Council (ERC) [256270] Funding Source: European Research Council (ERC)
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We demonstrate that discrete three-dimensional (3D) DNA components can specifically self-assemble in solution on the basis of shape-complementarity and without base pairing. Using this principle, we produced homo-and heteromultimeric objects, including micrometer-scale one- and two-stranded filaments and lattices, as well as reconfigurable devices, including an actuator, a switchable gear, an unfoldable nanobook, and a nanorobot. These multidomain assemblies were stabilized via short-ranged nucleobase stacking bonds that compete against electrostatic repulsion between the components' interfaces. Using imaging by electron microscopy, ensemble and single-molecule fluorescence resonance energy transfer spectroscopy, and electrophoretic mobility analysis, we show that the balance between attractive and repulsive interactions, and thus the conformation of the assemblies, may be finely controlled by global parameters such as cation concentration or temperature and by an allosteric mechanism based on strand-displacement reactions.
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