Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 24, Issue 21, Pages 3337-3349Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E13-05-0253
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Funding
- Wellcome Trust Institutional Strategic Support Fund [097828/Z/11/Z]
- Wellcome Trust [097828/Z/11/Z] Funding Source: Wellcome Trust
- Cancer Research UK [15675] Funding Source: researchfish
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In humans, MOZART1 plays an essential role in mitotic spindle formation as a component of the gamma-tubulin ring complex. We report that the fission yeast homologue of MOZART1, Mzt1/Tam4, is located at microtubule-organizing centers (MTOCs) and coimmunoprecipitates with gamma-tubulin Gtb1 from cell extracts. We show that mzt1/tam4 is an essential gene in fission yeast, encoding a 64-amino acid peptide, depletion of which leads to aberrant microtubule structure, including malformed mitotic spindles and impaired interphase microtubule array. Mzt1/Tam4 depletion also causes cytokinesis defects, suggesting a role of the gamma-tubulin complex in the regulation of cytokinesis. Yeast two-hybrid analysis shows that Mzt1/Tam4 forms a complex with Alp6, a fission yeast homologue of gamma-tubulin complex protein 3 (GCP3). Biophysical methods demonstrate that there is a direct interaction between recombinant Mzt1/Tam4 and the N-terminal region of GCP3(Alp6). Together our results suggest that Mzt1/Tam4 contributes to the MTOC function through regulation of GCP3(Alp6).
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