Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 23, Issue 18, Pages 3602-3611Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E11-08-0666
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Funding
- National Institutes of Health [DK44650]
- National Cancer Institute [CA104125]
- Optical Morphology Core of the Mayo Clinic Center for Cell Signaling in Gastroenterology [P30DK84567]
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Ubiquitination of the epidermal growth factor receptor (EGFR) by cbl and its cognate adaptor cbl-interacting protein of 85 kDa (CIN85) is known to play an essential role in directing this receptor to the lysosome for degradation. The mechanisms by which this ubiquitin modification is regulated are not fully defined, nor is it clear where this process occurs. In this study we show that EGFR activation leads to a pronounced src-mediated tyrosine phosphorylation of CIN85 that subsequently influences EGFR ubiquitination. Of importance, phospho-CIN85 interacts with the Rab5-positive endosome, where it mediates the sequestration of the ubiquitinated receptor into multivesicular bodies (MVBs) for subsequent degradation. These findings provide novel insights into how src-kinase-based regulation of a cbl adaptor regulates the fate of the EGFR.
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