Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 21, Issue 9, Pages 1462-1469Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E09-10-0885
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Funding
- Wellcome Trust
- European Framework 6 SIROCCO consortium fund
- Medical Research Council
- RUBICON European Union
- BBSRC
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Key components of the miRNA-mediated gene regulation pathway are localized in cytoplasmic processing bodies (P-bodies). Mounting evidence suggests that the presence of microscopic P-bodies are not always required for miRNA-mediated gene regulation. Here we have shown that geldanamycin, a well-characterized HSP90 inhibitor, abolishes P-bodies and significantly reduces Argonaute and GW182 protein levels but does not affect the miRNA level and the efficiency of miRNA-mediated gene repression; however, it significantly impairs siRNA loading and the efficacy of exogenous siRNA. Our data suggests that HSP90 protein chaperones Argonautes before binding RNA and may facilitate efficient loading of small RNA.
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