Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 21, Issue 13, Pages 2338-2354Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E10-01-0028
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Funding
- National Institutes of Health National (NIH) [P30 NS-047101, DK-80506, CA-100768, DK-I7780, T32 DK-07202]
- Burroughs Wellcome Fund
- American Gastroenterology Association FDN
- Basque Government [BFI06.300]
- Susan G. Komen [KG080079]
- UCSD McNair Scholarship Program
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Cells respond to growth factors by either migrating or proliferating, but not both at the same time, a phenomenon termed migration-proliferation dichotomy. The underlying mechanism of this phenomenon has remained unknown. We demonstrate here that G alpha(i) protein and GIV, its nonreceptor guanine nucleotide exchange factor (GEF), program EGF receptor (EGFR) signaling and orchestrate this dichotomy. GIV directly interacts with EGFR, and when its GEF function is intact, a G alpha(i)-GIV-EGFR signaling complex assembles, EGFR autophosphorylation is enhanced, and the receptor's association with the plasma membrane (PM) is prolonged. Accordingly, PM-based motogenic signals (PI3-kinase-Akt and PLC gamma 1) are amplified, and cell migration is triggered. In cells expressing a GEF-deficient mutant, the G alpha i-GIV-EGFR signaling complex is not assembled, EGFR autophosphorylation is reduced, the receptor's association with endosomes is prolonged, mitogenic signals (ERK 1/2, Src, and STAT5) are amplified, and cell proliferation is triggered. In rapidly growing, poorly motile breast and colon cancer cells and in noninvasive colorectal carcinomas in situ in which EGFR signaling favors mitosis over motility, a GEF-deficient splice variant of GIV was identified. In slow growing, highly motile cancer cells and late invasive carcinomas, GIV is highly expressed and has an intact GEF motif. Thus, inclusion or exclusion of GIV's GEF motif, which activates G alpha i, modulates EGFR signaling, generates migration-proliferation dichotomy, and most likely influences cancer progression.
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