Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 20, Issue 24, Pages 5260-5275Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E09-05-0377
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Funding
- Human Frontier Science Programme Long-Term fellowship [LT00519/2003]
- Wellcome Trust [R41067, 067433]
- EMBL Predoctoral Fellowship
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The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore complex and is localized to kinetochores in mitosis. Here we report that Seh1, a component of the Nup107-160 complex, functions in chromosome alignment and segregation by regulating the centromeric localization of Aurora B and other chromosome passenger complex proteins. Localization of CENP-E is not affected by Seh1 depletion and analysis by electron microscopy showed that microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired Aurora B localization, which results in severe defects in biorientation and organization of the spindle midzone and midbody. Our results indicate that a major function of the Nup107 complex in mitosis is to ensure the proper localization of the CPC at the centromere.
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