Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 19, Issue 3, Pages 945-956Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E07-09-0967
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Funding
- Centre National de la Recherche Scientifique
- INSERM
- Association pour la recherche sur le cancer [3118, 3327]
- Agence Nationale pour la Recherche sur le SIDA
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Transforming growth factor beta(TGF-beta) has a strong impact on liver development and physiopathology, exercised through its pleiotropic effects on growth, differentiation, survival, and migration. When exposed to TGF-beta, the mhAT3F cells, immortalized, highly differentiated hepatocytes, maintained their epithelial morphology and underwent dramatic alterations of adhesion, leading to partial or complete detachment from a culture plate, followed by readhesion and spreading. These alterations of adhesive behavior were caused by sequential changes in expression of the alpha 5 beta 1 integrin and of its ligand, the fibronectin. The altered specificity of anchorage to the extracellular matrix gave rise to changes in cells' collective motility: cohorts adhering to fibronectin maintained a persistent, directional motility, with ezrin-rich pathfinder cells protruding from the tips of the cohorts. The absence of adhesion to fibronectin prevented the appearance of polarized pathfinders and lead to random, oscillatory motility. Our data suggest a novel role for TGF-beta in the control of collective migration of epithelial cohorts.
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