Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 19, Issue 6, Pages 2553-2565Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E07-09-0862
Keywords
-
Categories
Funding
- National Institutes of Health [GM052454-09]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM052454] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Alignment of the mitotic spindle along a preformed axis of polarity is crucial for generating cell diversity in many organisms, yet little is known about the role of the endomembrane system in this process. RAB-11 is a small GTPase enriched in recycling endosomes. When we depleted RAB-11 by RNAi in Caenorhabditis elegans, the spindle of the one-cell embryo failed to align along the axis of polarity in metaphase and underwent violent movements in anaphase. The distance between astral microtubules ends and the anterior cortex was significantly increased in rab-11(RNAi) embryos specifically during metaphase, possibly accounting for the observed spindle alignment defects. Additionally, we found that normal ER morphology requires functional RAB-11, particularly during metaphase. We hypothesize that RAB-11, in conjunction with the ER, acts to regulate cell cycle-specific changes in astral microtubule length to ensure proper spindle alignment in Caenorhabditis elegans early embryos.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available