Journal
SCIENCE
Volume 349, Issue 6255, Pages 1525-1529Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aac9283
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Funding
- European Union [266025]
- European Research Council under the European Union [638271]
- UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/K0017802/1]
- Royal Society
- BBSRC [BB/M017702/1, BB/K00199X/1] Funding Source: UKRI
- EPSRC [EP/J020192/1] Funding Source: UKRI
- European Research Council (ERC) [638271] Funding Source: European Research Council (ERC)
- Biotechnology and Biological Sciences Research Council [BB/M017702/1, BB/K00199X/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J020192/1] Funding Source: researchfish
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alpha-Chiral amines are key intermediates for the synthesis of a plethora of chemical compounds at industrial scale. We present a biocatalytic hydrogen-borrowing amination of primary and secondary alcohols that allows for the efficient and environmentally benign production of enantiopure amines. The method relies on a combination of two enzymes: an alcohol dehydrogenase (from Aromatoleum sp., Lactobacillus sp., or Bacillus sp.) operating in tandem with an amine dehydrogenase (engineered from Bacillus sp.) to aminate a structurally diverse range of aromatic and aliphatic alcohols, yielding up to 96% conversion and 99% enantiomeric excess. Primary alcohols were aminated with high conversion (up to 99%). This redox self-sufficient cascade possesses high atom efficiency, sourcing nitrogen from ammonium and generating water as the sole by-product.
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