Journal
MOLECULAR AND CELLULAR PROBES
Volume 27, Issue 1, Pages 68-70Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2012.10.001
Keywords
Listeria spp.; Listeria monocytogenes; Serogrouping; Real-time PCR; Molecular epidemiology
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A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L monocytogenes. (C) 2012 Elsevier Ltd. All rights reserved.
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