4.4 Article

Rapid on-site detection of Acidovorax citrulli by cross-priming amplification

Journal

MOLECULAR AND CELLULAR PROBES
Volume 26, Issue 4, Pages 175-176

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2012.03.010

Keywords

Acidovorax citrulli; Cross-priming amplification; Detection

Funding

  1. special fund for inspection and quarantine scientific Research in the Public Interest [200810632, 201010256, 2012IK294]
  2. special Fund for Agroscientific Research in the Public Interest [201003066]

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Cross-priming amplification (CPA) for Acidovorax citrulli detection was evaluated in this study. The sensitivity of CPA assay for pure bacterial culture was 3.7 x 10(3) CFU/ml. Bacteria on naturally infected watermelon seeds were detected using CPA assay, suggesting this method is suitable for A. citrulli on-site detection from watermelon seeds. (C) 2012 Elsevier Ltd. All rights reserved.

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