4.4 Article

Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR

Journal

MOLECULAR AND CELLULAR PROBES
Volume 24, Issue 6, Pages 401-402

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2010.07.002

Keywords

Complement C3; Polymorphisms; Tetra primer ARMS PCR

Funding

  1. Ministero della Salute (Italy)
  2. Compagnia di San Paolo (Torino Italy)

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Complement C3 slow and fast allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid reproducible and cost-effective method to genotype both complement C3 slow and fast alleles by a single tube reaction (C) 2010 Published by Elsevier Ltd

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