Journal
SCIENCE
Volume 348, Issue 6232, Pages 344-347Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa0445
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Funding
- NIH [GM065367]
- NSF (Physics Frontier Center) [0822613, MCB 09-52442]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0952442] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Physics [1430124] Funding Source: National Science Foundation
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Conformational control of biomolecular activities can reveal functional insights and enable the engineering of novel activities. Here we show that conformational control through intramolecular cross-linking of a helicase monomer with undetectable unwinding activity converts it into a superhelicase that can unwind thousands of base pairs processively, even against a large opposing force. A natural partner that enhances the helicase activity is shown to achieve its stimulating role also by selectively stabilizing the active conformation. Our work provides insight into the regulation of nucleic acid unwinding activity and introduces a monomeric superhelicase without nuclease activities, which may be useful for biotechnological applications.
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