Journal
MOLECULAR AND CELLULAR NEUROSCIENCE
Volume 46, Issue 1, Pages 32-44Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2010.07.015
Keywords
Dorsal root ganglion; Transcription factor; High content analysis; Screen; Systems biology; Cerebellar granule neuron; STAT3
Categories
Funding
- Miami Project to Cure Paralysis
- Buoniconti Fund [DOD W81XWH-05-1-0061]
- Paralyzed Veterans of America Research Foundation [2396]
- NIH [HD057632, NS059866]
- Lois Pope LIFE Scholar award
Ask authors/readers for more resources
Neurons in the peripheral nervous system (PNS) display a higher capacity to regenerate after injury than those in the central nervous system, suggesting cell specific transcriptional modules underlying axon growth and inhibition. We report a systems biology based search for PNS specific transcription factors (TFs). Messenger RNAs enriched in dorsal root ganglion (DRG) neurons compared to cerebellar granule neurons (CGNs) were identified using subtractive hybridization and DNA microarray approaches. Network and transcription factor binding site enrichment analyses were used to further identify TFs that may be differentially active. Combining these techniques, we identified 32 TFs likely to be enriched and/or active in the PNS. Twenty-five of these TFs were then tested for an ability to promote CNS neurite outgrowth in an overexpression screen. Real-time PCR and immunohistochemical studies confirmed that one representative TF, STAT3, is intrinsic to PNS neurons, and that constitutively active STAT3 is sufficient to promote CGN neurite outgrowth. (C) 2010 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available