Journal
MOLECULAR AND CELLULAR NEUROSCIENCE
Volume 46, Issue 1, Pages 262-271Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2010.09.009
Keywords
Interleukin-1 beta; Bradykinin receptor 2; G protein-coupled receptor kinase 2; Dorsal root ganglion; Receptor internalization
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Funding
- Deutsche Forschungsgemeinschaft (DFG)
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In dorsal root ganglion sections numerous small-to medium-sized neurons were found to exhibit extensive colocalization of the bradykinin receptor 2, the interleukin-1 receptor 1 and G protein-coupled receptor kinase 2. Application of bradykinin to cultured DRG neurons caused substantial internalization of the bradykinin 2 receptor which significantly reduced the responsiveness of DRG neurons to a second application of bradykinin. Such an internalization was not observed in DRG neurons which were exposed to long-term pretreatment with interleukin-1 beta. The long-term incubation with interleukin-1 beta on its own did neither change the proportion of neurons which expressed the bradykinin 2 receptor in the cytoplasma nor the proportion of neurons expressing the bradykinin 2 receptor in the membrane but it reduced the proportion of neurons expressing G protein-coupled receptor kinase 2, an enzyme which facilitates the internalization of G protein-coupled receptors. These results show that interleukin-1 beta maintains the responsiveness of DRG neurons to bradykinin in the long-term range, and they suggest that the downregulation of G protein-coupled receptor kinase 2 could be a cellular mechanism involved in this interleukin-1 beta effect. (C) 2010 Elsevier Inc. All rights reserved.
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