Cell-specific regulation of the pro-survival Brn-3b transcription factor by microRNAs

We have previously shown that the Brn 3b transcription factor is subjected to post-transcriptional gene regulation by specific microRNAs (mir 23 and mir-214) in the ND7 and SHSY-5Y neuronal cell lines (Calissano et al 2007) As Brn 3b plays an essential role in the survival of retinal ganglion cells in the rat (Erkman et al 1996 Can et al 1996 Can et al 1999 Erkman et al 2000) we wanted to investigate whether mir 23 and mir-214 are expressed and target Brn 3b mRNA in a retinal ganglion cell line (RGC 5) thus potentially killing the cells expressing it Here we show that possibly due to its pro-survival role Brn-3b is protected from degradation by microRNAs in RGC 5 cells in contrast to its fate in other cell types This seems to be accomplished by i) the lack of expression of one of the two microRNAs targeting its 3'UTR and by ii) the requirement of at least two distinct microRNAs to mediate its down-regulation in retinal ganglion cells We speculate that this mechanism could have a widespread role in the regulation of mRNAs encoding for essential proteins (C) 2010 Elsevier Inc All rights reserved