Neuronal Ca2+ sensor VILIP-1 leads to the upregulation of functional α4β2 nicotinic acetylcholine receptors in hippocampal neurons

The neuronal Ca2+-sensor protein VILIP-1, known to affect clathrin-dependent receptor trafficking, has been shown to interact with the cytoplasmic loop of the alpha 4-subunit of the alpha 4 beta 2 nicotinic acetylcholine receptor :nAChR), which is the most abundant nAChR subtype with high-affinity for nicotine in the brain. The alpha 4 beta 2 nAChR is crucial for nicotine addiction and the beneficial effects of nicotine on cognition. Its dysfunction has been implicated in frontal lobe epilepsy, Alzheimer's disease and schizophrenia. Here we report that overexpression of VILIP-1 enhances ACh responsiveness, whereas siRNA against VILIP-1 reduces alpha 4 beta 2 nAChR currents of hippocampal neurons. The underlying molecular mechanism likely involves enhanced constitutive exocytosis of alpha 4 beta 2 nAChRs mediated by VILIP-1. The two interaction partners co-localize in a Ca2+-dependent manner with syntaxin-6, a Golgi-SNARE protein involved in trans-Golgi membrane trafficking. Thus, we speculate that regulation of VILIP-1-expression might modulate Surface expression of ligand-gated ion channels, such as the alpha 4 beta 2 nAChRs, possibly comprising a novel form of physiological upregulation of ligand-gated ion channels. (C) 2008 Elsevier Inc. All rights reserved.