Journal
MOLECULAR AND CELLULAR NEUROSCIENCE
Volume 38, Issue 4, Pages 518-525Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2008.04.013
Keywords
olfactory sensory neuron; neuronal death; culture; bulbectomy; jun N-terminal kinase
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Funding
- NIGMS NIH HHS [1R01GM085081, R01 GM085081, R01 GM085081-01] Funding Source: Medline
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Olfactory sensory neurons (OSNs) represent a unique population of neurons in which death and regeneration are ongoing throughout adulthood, a feature that makes them an attractive model cell type for the investigation of neuronal death. However, the mechanism by which OSNs die remains elusive. Therefore, we developed a culture system for studying pathways involved in OSN death. Here, we show that inhibition of transcription or translation, by actinomycin D or cycloheximide, respectively, suppresses pathways leading to death, prolonging the survival of OSNs in culture. We discovered that caspase activity and jun N-terminal kinase (JNK) signaling both play a role in OSN death, and inhibition of JNK activity suppresses effector caspase (caspase-3) activation. Results from studies in culture were confirmed in vivo, in a mouse bulbectomy-induced OSN death model. These findings provide new insights into the nature of OSN death and a means of studying OSNs in vitro. (C) 2008 Elsevier Inc. All rights reserved.
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