Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 384, Issue 1-2, Pages 23-31Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2013.12.015
Keywords
RFamide-related peptide; GnIH; GPR147; Hypothalamus; Stress; Glucocorticoids
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Funding
- Canadian Institutes for Health Research (CIHR)
- Canada Foundation for Innovation
- Canada Research Chairs Program
- Ontario Graduate Scholarship (OGS)
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A novel RFamide peptide, gonadotropin-inhibitory hormone (GnIH) has emerged as a modulator of avian reproduction. However, the functional role of the mammalian homologue, RFRP-3 remains poorly understood. The RFRP-3 neuronal circuit is influenced by the stress axis. However, whether the Rfrp gene is under direct glucocorticoid (GC)-mediated transcriptional regulation, in the presence and absence of the gonadal steroid, 17 beta-estradiol, is unknown. We investigated the regulation of the Rfrp (GnIH) and Gpr147 (GnIH-R) transcripts by steroids in a novel hypothalamic Rfrp-expressing cell model, rHypoE-23. The GC agonist, dexamethasone increased Rfrp and Gpr147 mRNA levels. Dexamethasone acted directly on the nuclear GC receptor (GR) to mediate GC-dependent transcriptional changes, independently of de novo protein synthesis. 17 beta-estradiol had no significant effect on Rfrp or Gpr147 biosynthesis in these neurons. This suggests that Rfrp-expressing neurons serve as potential upstream mediators of stress-induced effects through GR-dependent mechanisms. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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